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From: Leander, TX
Region: Southwest
Topic: Propagation
Title: Rhizobium inoculant for Leucaena and othr leguminous tree seedlings
Answered by: Nan Hampton and Joe Marcus
Research in Australia has cited two strains of Rhizobium that are effective with Luecaena. In acid soils Strain CB3060 (TAL1145) was most effective in N2 fixation. This is cited in Tolerance of Leucaena to Acid Soil Conditions by F.P.C.Blarney and E. M. Hutton, p.84. In the article "Establishment and Early Growth of Leucaena" C. M. Piggin et al., p. 87, report strain CB81 was found reliable and strain NGR8 less reliable. They also report (p. 88) CB3060 (TAL1145) is good in acidic soils as well as more neutral soils.
Unfortunately, there are few sources for obtaining Rhizobium cultures. I did find one source, Becker Underwood in Australia with worldwide production and marketing, for purchase of CB3060. The USDA has a Rhizobium Germplasm Collection that provides free cultures for research but they do not have a database of strains available. You can see a list of other bacterial culture collections worldwide.
For a good discussion of seed treatment and nitrogen fixation see Agroforestry for the Pacific Technologies from the U. S. Forest Service and also Nitrogen Fixing Tree Start-up Guide from Agroforestry.com.
You can also check with the Williamson County Extension Office of the Texas Agricultural Extension Service and see if they know of the availability of Rhizobium strains.
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